Antioxidant properties of ethanolic and aqueous Cinnamon extracts against liver injury in rats.
Keywords:
Cinnamon, liver injury, oxidative stress, ratsAbstract
The hepatoprotective activity of aqueous and ethanolic extracts of cinnamon were investigated against carbon tetrachloride (CCl4) induced lipid peroxidation and hepatic injury in rats. The elevated serum AST and ALT enzymatic activities induced by CCl4 were restored towards normalization significantly by orally administrated 200 mg/kg of either extracts once daily for 7 days as compared to non treated rats. There was a significant elevation in the level of liver malondialdhyde (MDA) while the activities of antioxidant enzymes superoxide dismutase and catalase (SOD and CAT) were significantly decreased in CCl4 intoxicated rats. The results obtained indicated that ethanolic extract have potent hepatoprotective action more than water extract against CCl4 by lowering the MDA level and elevate the antioxidants enzymes activities (SOD and CAT). It is concluded that, the possible mechanism of this activity may be due to free radicalscavenging polyphenols compounds.
References
Murcia MA, Egea I, Romojaro F, Parras P,
Jimenez AM, Martinez-Tome M. Antioxidant
evaluation in dessert spices compared with
common food additives. Influence of irradiation
procedure. Journal of Agricultural and Food
Chemistry. 2004. 52, 1872–1881.
Mancini-Filho J, Van-Koiij A. Antioxidant
activity of cinnamon (Cinnamomum Zeylanicum,
Breyne) extracts. Bollettino Chimico
Farmaceutico. 1998. 137, 443–447.
Peschel W, Sanchez-Rabaneda F, Dickmann W,
Plesehen A, Gartiza I, Jimenez D, LamuelaRaventos R, Buxaderas S, Codina C, An
Industrial approach in the search of natural
antioxidants from vegetables and fruit wastes.
Food Chemistry. 2006. 97, 137–150.
Reitman S, Frankel S. A colorimetric method for
the determination of serum glutamate
oxaloacetate transaminase. American Journal of
Clinical Pathology 1957. 28, 53–56.
Rekka, E, Kourounakis, P.Shahidi, F., Janitha,
P.K., Wanasundara, P.D., Phenolic antioxidants.
Critical Reviews in Food Science and Nutrition
32, 67–103.
Shaughnessy DT, Setzer RW, & DeMarini DM.
The antimutagenic effect of vanillin and
cinnamaldehyde on spontaneous mutation in
Salmonella TA 104 is due to a reduction in
mutations at GC but not AT sites. Mutation
Research 2001. 480/481, 55–69.
Smna KA. Colourimetric assay of catalase.
Analytical Biochemistry. 1972, 47, 389–394.
Thabrew MI, Joice PDTM, Rajatissa WA,
Comparative study of efficacy of Paetta indica
and Osbeckia octandra in the treatment of liver
dysfunction. Planta Medica. 1987. 53, 239–241.
Vekiari SA, Oreopoulou V, Tzia C,
Thomopoulos, C. D. Oregano flavonoids as lipid
antioxidants. Journal of the American Oil
Chemists Society. 1993. 70, 483–487.
Yagi K, Rastogi R. Assay for lipid peroxides in
animal tissues by thiobarbituric acid reaction.
Analytical Biochemistry. 1979 95, 351–358.
Anderson RA, Broadhurst CL. Isolation and
characterization of polyphenol type-A polymers
from cinnamon with insulin-like biological
activity. Journal of Agricultural and Food
Chemistry. 2004. 52, 65–70.
Avijeet Jain , Manish Soni, Lokesh Deb,
Anurekha Jain, S.P. Rout, V.B. Gupta, K.L.
Krishna Antioxidant and hepatoprotective
activity of ethanolic and aqueousextracts of
Momordica dioica Roxb. Leaves. Journal of
Ethnopharmacology. 2008. 115 61–66.
Bhathal, PS, Rose NR, Mackay IR. Whittingham,
S., Strain differences in mice in
carbon tetrachloride-induced liver injury. British
Journal of Experimental Pathology. 1983. 64,
–533.
Chatterjee TK. Medicinal Plants with
Hepatoprotective Properties in Herbal Opinions,
vol. III. Books and Allied (P) Ltd., Calcutta,
135.
Chattopadhyay RR. Possible mechanism of
hepatoprotective activity of Azadirachta indica
leaf extract. Part II. Journal of
Ethnopharmacology. 2003, 89, 217–219.
Gurdip Singh , Sumitra Maurya , M.P.
deLampasona , Cesar A.N. Catalan.A
comparison of chemical, antioxidant and
antimicrobial studies of cinnamon leaf and bark
volatile oils, oleoresins and their constituents
Food and Chemical Toxicology. 2007 45. 1650–
James GWL, Pickering RW. The protective
effect of a novel compound RU-18492 on
galactosamine induced hepaptotoxicity in rats.
Drug Research. 1976. 26, 2197–2199.
Lan Su, Jun-Jie Yin, Denys Charles , Kequan
Zhou ,Jeffrey Moore Liangli .Total phenolic
contents, chelating capacities, and radicalscavenging properties of black peppercorn,
nutmeg, rosehip, cinnamon and oregano leaf
Food Chemistry. 2007.100 990–997
