Structure-guided Modeling of Arachidonate 15-lipoxygenase B from H. sapiens

Abstract

15-Lipoxygenase is a highly regulated lipid–peroxidating enzyme whose expression and arachidonic acid metabolites are implicated in several important inflammatory conditions. In this scenario, we had performed modeling of arachidonate 15-lipoxygenase B, an enzyme involved in Leucotriene biosynthesis. We wish to contribute to the structural knowledge of arachidonate 15-lipoxygenase B from Homo sapiens. In this pursuit, 3D model of arachidonate 15-lipoxygenase B has been established by homology modeling, choosing the Revised structure of rabbit reticulocyte 15S-lipoxygenase with PDB code 2P0M and Crystal structure of Delta 413-417:GS LOX with PDB code 3FG1 as templates by the MODELLAR9v7 program. The model was extensively validated using protein structure checking tools: PROCHECK, WHAT IF and ProSA. The developed model was analyzed through domain analysis for the presence of various domains. Secondary structural conformations for the developed model were determined using PDBSUM. This study suggests use of this 3D structure as a target of new drugs that act on multidrug resistant varieties of Homo sapiens.